[1]国巍,燕红,裴蕾.可降解木质纤维素的双菌共生菌丝球的制备[J].哈尔滨理工大学学报,2018,(04):127-132.[doi:10.15938/j.jhust.2018.04.024]
 GUO Wei,YAN Hong,PEI Lei. Study on Immobilization Method of Twospecies Mycelial Pellet for Degradation of Lignocellulose[J].哈尔滨理工大学学报,2018,(04):127-132.[doi:10.15938/j.jhust.2018.04.024]
点击复制

可降解木质纤维素的双菌共生菌丝球的制备
()
分享到:

《哈尔滨理工大学学报》[ISSN:1007-2683/CN:23-1404/N]

卷:
期数:
2018年04期
页码:
127-132
栏目:
材料科学与工程
出版日期:
2018-08-25

文章信息/Info

Title:
 Study on Immobilization Method of Twospecies 
Mycelial Pellet for Degradation of Lignocellulose
作者:
国巍12燕红12裴蕾12
1.哈尔滨理工大学 化学与环境工程学院,黑龙江 哈尔滨 150040;
2.黑龙江省高校绿色化工技术重点实验室,黑龙江 哈尔滨 150040
Author(s):
 GUO Wei12YAN Hong12PEI Lei12
 1.School of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China; 
2.Key Laboratory of Green Chemical Technology of College of Heilongjiang Province, Harbin 150040, China
关键词:
关键词:菌丝球木质素降解菌纤维素降解菌木质素酶纤维素酶
Keywords:
 Keywords:mycelia pelletslignindegrading fungus cellulosedegrading bacteria ligninase cellulase
分类号:
Q9399
DOI:
10.15938/j.jhust.2018.04.024
文献标志码:
A
摘要:
 摘要:以共固定化菌丝球的产酶能力作为指标,将木质素降解菌G13和纤维素降解菌X1012进行共固定,探讨了X1012的接入时间、接种量、pH值、摇床的转速和温度对菌丝成球和产木质纤维素酶的影响。结果表明,当G13与X1012同时接入、纤维素降解菌的接种量为20mL(100mL培养基),培养基的pH值为5、摇床转速为160r/min、温度为28℃时,锰过氧化物酶、漆酶、木质素过氧化物酶、纤维素酶和半纤维素酶的酶活达到最高,分别为31246U/L、16048U/L、157528U/L、197063U/L和21581U/L。X1012与G13在共固定过程中,具有互补的性能,在产酶的过程中发挥了协同的作用,促进了木质素降解酶的分泌。所获得的双菌共生菌丝球的球体为白色,表面光滑,大小均匀,具有一定的机械强度,能够满足实践应用的需求。
Abstract:
 Abstract:In our research, enzyme production ability of immobilization mycelial pellet was used as an index, the immobilization mycelial pellet was immobilized with lignindegrading fungus G13 and cellulosedegrading bacteria X1012 The influence of five factors (addition time, inoculation amount of cellulosedegrading bacteria, pH, temperature and rotational speed) to Mycelium pellet, ligninase production and cellulase production were studied The results indicated that X1012 and G13 were added simultaneously and the inoculation amount was 20%, pH was 5, culture temperature was 28℃, rotational speed was 160 r/min, the manganese(MnP); laccase(Lac); lignin peroxidase(Lip); cellulose; hemicellulose were 31246U/L;16048U/L;157528U/L;197063U/L;21581U/L, respectively X1012 and G13 have the complementary advantage on the immobilization process, develope the synergy effects and promote the production of ligninase The immobilization mycelial pellet was white, smooth surface, uniform size and good elasticity, which can satisfy practical needs

参考文献/References:

 [1]柳荣展, 王广建 白腐菌在制浆造纸工业中的应用进展[J]. 青岛大学学报, 2001, 16(3): 46-50
[2]DU Xueyu, MARTA Perezbozda, CARMEN Fernandez, et al Analysis of Lignincarbohydrate and Ligninlignin Linkages after Hydrolase Treatment of Xylanlignin[J]. Glucomannanlignin and Glucanlignin Complexes from Spruce Wood Planta, 2014(239):1079-1090
[3]吴香波,谢益民造纸废水微生物处理现状和发展[J]湖北造纸,2008(3):7-10
[4]MUNOZ G, VALENCIA C,VALDERRUTEN N, et al Extraction of Chitosan from Aspergillus Niger Mycelium and Synthesis of Hydrogels for Controlled Release Ofbetahistine[J]. Reactive & Functional Polymers, 2015(91):1-10
[5]MUNOZ G, VALENCIA C,VALDERRUTEN N, et al Extraction of Chitosan from Aspergillus Niger Mycelium and Synthesis of Hydrogels for Controlled Release Ofbetahistine[J]. Reactive & Functional Polymers, 2015( 91):1-10
[6]JP2〗张丽霞,张虹,卞立红等 产表面活性剂菌株(QSM2)的筛选及其特性研究[J]哈尔滨理工大学学报,2016,21(4):65-69
[7]俞丽平生化和深度处理法处理造纸中段废水[J]环保与节能,2012,43(1): 71-74
[8]刘荣荣,石光辉,吴春笃固定化微生物技术研究进展及其在印染废水处理中的应用[J]印染助剂,2014,31(3):1-5
[9]黄勋娟,刁宁宁,张建国黑曲霉菌丝球的形成及应用研究综述[J]食品与发酵工业,2014,40(11):171-176
[10]KG*2〗ZHOU YING, DU JIANXIN, TSAO GEORGE T Mycelial Pellet Formation by Rhizopus Oryzae ATCC 20344[J] Applied Biochemistry and Biotechnology, 2000(84/86): 779-789
[11]KG*2〗LIU YUANSHUAI, WU JIANYONG Effects of Tween 80 and pH on Mycelial Pellets and Exopolysaccharide Production in Liquid Culture of a Medicinal Fungus[J] Journal of Industrial Microbiology and Biotechnology, 2012(39): 623-628
[12]KG*2〗ZHANG SI, LI ANG, CUI DI, et al Performance of Enhanced Biological SBR Process for Aniline Treatment by Mycelial Pellet as Biomass Carrier[J]Bioresource Technology, 2011(102): 4360-4365
[13]KG*2〗PRIEGNITZ BERTEWALD, WARGENAU ANDREAS, BRANDT ULRIKE, et al The Role of Initial Spore Adhesion in Pellet and Biofilm Formation in Aspergillus Niger[J] Fungal Genetics and Biology, 2012(49): 30-38
[14]KG*2〗王宝娥,周康群,麦霖霞,等烟曲霉菌丝球吸附富里酸的性能及相互作用[J]环境科学与技术,2013,36(5):38-42
[15]KG*2〗程子彰,杨洲平,胡容,等粗毛栓菌菌丝球非灭菌条件下对12种染料的脱色研究[J]菌物学报,2012,31(6):878-889
[16]KG*2〗张燕,刁宁宁,黄勋娟,等发酵罐中利用黑曲霉菌丝球处理豆制品废水的研究[J]工业微生物,2016,46(1):22-25
[17]KG*2〗董学卫,朱启忠,于秀敏,等纤维素酶固定化研[J]哈尔滨理工大学学报,2008,13(5):118-112
[18]KG*2〗HUANG Danlian, ZENG Guangming, FENG Chongling, et al Mycelial Growth and Solidstate Fermentation of Lignocellulosic Waste by Whiterot Fungus Phanerochaete Chrysosporium under Lead Stress[J] Chemosphere, 2010(81): 1091-1097
[19]KG*2〗YU MAN,ZENG GUANGMING, CHEN YAONING, et al Influence of Phanerochaete Chrysosporium on Microbial Communities and Lignocellulose Degradation During Solidstate Fermentation of Rice Straw[J] Process Biochemistry, 2009(44): 17-22
[20]KG*2〗CHAO YAPENG, SINGH DEEPAK, YU LIANG, et al Secretome Characteristics of Pelletized Trichoderma Reesei and Cellulase Production[J] World Journal of Microbiology and Biotechnology, 2012(28): 2635-2641
[21]KG*2〗WANG QI, GUAN YIXIN, YAO SHANJING, et al Microparticle Formation of Sodium Cellulose Sulfate Using Supercritical Fluid Assisted Atomization Introduced by Hydrodynamic Cavitation Mixer[J] Chemical Engineering Journal, 2010(159): 220-229
[22]KG*2〗NYMAN J,LACINTRA MG, WESTMAN JO, et al Pellet Formation of Zygomycetes and Immobilization of Yeast[J]. New Biotechnology, 2013, 30(5): 516-522

备注/Memo

备注/Memo:
 基金项目:国家自然科学基金(21776054);黑龙江省教育厅科学技术研究面上项目(11541067)
更新日期/Last Update: 2018-10-25