[1]冯茜,国巍,燕红,等. 木质素降解菌的分离筛选及菌丝成球条件优化[J].哈尔滨理工大学学报,2019,(01):138-144.[doi:10.15938/j.jhust.2019.01.023]
 FENG Qian,GUO Wei,et al. Isolation and Screening of the Lignindegrading Fungusand Optimization of the Mycelium Pellet Cultivation Conditions[J].哈尔滨理工大学学报,2019,(01):138-144.[doi:10.15938/j.jhust.2019.01.023]
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 木质素降解菌的分离筛选及菌丝成球条件优化

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《哈尔滨理工大学学报》[ISSN:1007-2683/CN:23-1404/N]

卷:
期数:
2019年01期
页码:
138-144
栏目:
材料科学与工程
出版日期:
2019-08-06

文章信息/Info

Title:
 Isolation and Screening of the Lignindegrading Fungusand 
Optimization of the Mycelium Pellet Cultivation Conditions

作者:
 冯茜12国巍12燕红1 2
(1哈尔滨理工大学 化学与环境工程学院,黑龙江 哈尔滨 150040;
2黑龙江省高校绿色化工技术重点实验室,黑龙江 哈尔滨 150040)
Author(s):
 FENG Qian1 2GUO Wei12YAN Hong12
 (1School of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China;
2Key Laboratory of Green Chemical Technology of College of Heilongjiang Province, Harbin 150040, China)
关键词:
 关键词:木质素烟曲霉菌菌丝球条件优化生物载体
Keywords:
 Keywords:lignin Aspergillus fumigatus mycelia pelletscondition optimization biological carrier
分类号:
Q9399
DOI:
10.15938/j.jhust.2019.01.023
文献标志码:
A
摘要:
 摘要:为分离筛选出能高效降解木质素的丝状真菌,构建生物固定化载体,从造纸厂采集的污泥中分离筛选出一株高产木质素降解酶的真菌G13,其产锰过氧化物酶、漆酶和木质素过氧化物酶的活力分别为827、095和1355U/L。通过对菌丝成球影响因素的研究,得到最佳成球条件:18g/L的蔗糖和25g/L酒石酸铵分别为最佳碳源和氮源,培养基的pH值为5,将5mL菌悬液接种于100mL成球液体培养基(250mL锥形瓶)中,160r/min,30℃培养72h,得到的菌丝球球径最大,约为34mm,且表面光滑,弹性好,内部中空,是一种优良的生物固定化载体,能够满足实践应用的需求。
Abstract:
 Abstract:In order to isolate andscreen microorganisms that could degrade ligninefficiently, and then constructbiological immobilization carrier, there was 1 strain of high effcient lignindegrading fungi, named G13, isolated from the sludge samples collected by the paper mill, which the highest lingin enzyme activity of manganese peroxidase(MnP), laccase(Lac) and lignin peroxidase(LiP) were 827, 095 and 1355U/L, respectively Through the study of factors that can affect the pelletization, the best conditions are as follows: sucrose concentration of 18g/L and ammonium tartrate concentration of 25g/L were worked as the best carbon source and nitrogen source, respectively; the most appropriate medium pH was 5; 5mL of spore suspension was inoculated on 100mL of liquid medium forpelletization (in 250mL Erlenmeyer flask);the optimum temperature was 30℃; and the most suitable shaker speed was 160r/min Cultured under the most suitable growing conditions for 72h, the mycelium pellet formed by Aspergillus fumigatus G13 reached its maximum diameter of 34mm with smooth surface, excellent flexibility and hollow structure, which can be used as biological immobilization carrier as wellas meets the needs of practical application such as industrial wastewater treatment

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备注/Memo

备注/Memo:
 基金项目:国家自然科学基金(21776054)
更新日期/Last Update: 2019-03-26